Gene transfer lab report

The plate with ampicillin present with no plasmid DNA will not be able to grow colonies and will not be capable of bioluminescence. This experiments started with two tubes of uL E. Tube one just contained normal E.

Gene transfer lab report

DNA has all the blueprints for everything the cell will ever need to build. RNAs are assorted molecular machines that do all kinds of work in the cell.

They carry components from place to place, ratchet up the speed of operations, switch other machines on and off, and relay coded instructions from the nucleus out to the factory floor.

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The other product, proteins, are strong molecular machines that do things like hold the cell together and send signals to other cells. There are two parts to each assembly line: The process that builds RNAs is called transcription. This region is called a gene.

A protein machine inside the nucleus pries apart the weak bonds that hold the two strands of DNA together. This RNA strand now needs to fold up.

In the world of tiny cellular machines, shape determines function.

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Our transfer RNA is ready for action. It floats out of the nucleus and picks up an amino acid. This cell needs to make Thrombin, a protein machine which helps blood clot and make a scab.

Thrombin is just one of the tens of thousands of proteins your cells can make.

Gene transfer lab report

This mRNA carries the code for Thrombin out of the nucleus and onto the cellular factory floor. Our mRNA drifts until it runs into a ribosome, a protein-making machine.

The ribosome clamps down around the mRNA.

Gene transfer lab report

This process, translation, proceeds down the mRNA, creating a chain of bonded amino acids. So there you have it.

Unlike most factories, your cell uses just two processes to make tens of thousands of different molecular machines.Activity 4: Transformation of E.

coli using green fluorescent protein. Information for Teachers Hence, the natural function of a plasmid is to transfer genetic information vital to the survival of the bacteria.

It is this characteristic of plasmids that is exploited for use in transformation. Clean the lab . You need to upgrade your Flash Player. In Weedman’s genetic transformation experiment, we attempted to determine whether pGLO was a successful plasmid to transfer GFP into E.

Coli DNA. The first thing we did was obtain vinyl gloves to protect us from the E. Coli that we worked with throughout the entire lab.

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Jun 02,  · This feature is not available right now. Please try again later. Agrobacterium tumefaciens is a gram-negative rod-shaped bacteria (Jin, S.) closely related to nitrogen-fixing bacteria which dwell at root nodules in legumes. Unlike most other soil-dwelling bacteria, it infects the roots of plants to cause Crown Gall Disease (Jin, S.).

GTF A Gene Annotation Format